Application

Immunohistochemistry: 10-20 µg/mL on frozen sections of rat sciatic nerve perfused and counter-fixed with 4% paraformaldehyde (Stockli et al., 1991).

Western blotting: 10 µg/mL (Stockli et al., 1991)

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

This Anti-Ciliary Neurotrophic Factor Antibody, clone 4-68 is validated for use in WB, IH for the detection of Ciliary Neurotrophic Factor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified ciliary neurotrophic factor from rat sciatic nerve.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Liquid in 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide, pH 7.6.

Specificity

Ciliary neurotrophic factor. Detects a 22 kD band in Western blots of rat sciatic nerve extract.

Storage and Stability

Maintain at 2-8°C for up to 6 months. Avoid repeated freeze/thaw cycles.